In contrast, in a murine model of HFD-induced NASH, Cyp7b1 was down-regulated [89] and when Cyp7b1-/- mice were given the same diet, no significant differences were observed in relation to the NASH score, rendering the alternative pathway still ambiguously involved in MAFLD progression. This evidence concerns the gene CYP7B1 and metabolic dysfunction-associated steatohepatitis.