TP53 and cyclic hematopoiesis: To investigate whether neutrophil involvement is essential for cardiac toxicity in the t-CH model, mice underwent BMT to establish a VAF of 0.15 with heterozygous Trp53-deficient cells and were then treated with a course of Dox or saline in the presence of anti-Ly6G antibody, to deplete neutrophils, or an isotype control antibody (Figure 4A).