Since the tumor subtypes are different in gene expression and metabolism, we investigated the effect of glutamine depletion, GLUL expression, and GS abundance in the hepatoblastoma cell lines Huh-6 and HepT1, which differ in their mutations and, in contrast to the fetal-type HepG2, derive from tumors of the embryonal subtype (Crippa et al. 2017; Koch et al. 1999; Pietsch et al. 1996). Here, GLUL is linked to hepatoblastoma.