To explore the role of Sam68 in the effects of p300 depletion in CRC cells, particularly the phenomenon of resistance to butyrate, butyrate sensitive HCT-116 cell line (expresses p300) will be utilized, compared to butyrate resistant HCT-R cells that do not express p300, as well as the p300 KO HCT-116 line F5 that is partially butyrate resistant, and the p300 Rescue line in which p300 expression is restored to F5 cells, which, for the most part, restores butyrate sensitivity (Fig. 2) 44-47. The gene discussed is KHDRBS1; the disease is colorectal carcinoma.