To determine the impact of KTN1 on cell viability, this study continuously detected the relative viability of KTN1-KO cells and Huh7-WT cells within five days of knocking out KTN1. The results signified that cell viability was inhibited in KTN1-KO cells compared to Huh7-WT cells (Figure 5A), indicating that KTN1 could enhance the viability of HCC cells. The gene discussed is KTN1; the disease is hepatocellular carcinoma.