Along with these data, similar results were observed in MYPT1 phosphorylation as an indicator for ROCK activity and IRS1 serine 632/635 phosphorylation, which are ROCK’s phosphorylated sites (i.e., obesity decreased these parameters, but exercise increased them) (Figures 3, 4A). This evidence concerns the gene IRS1 and obesity due to melanocortin 4 receptor deficiency.