RT-PCR experiments performed in independent T-ALL confirmed that both Cdkn1a and Nr4a1 were up-regulated in response to either NFAT or CnB-1 inactivation (Fig 5D), validating at the molecular level the resemblance of CnB1 and NFAT loss of function phenotypes. This evidence concerns the gene PPP3R1 and acute lymphoblastic leukemia.