PPP3R1 and acute lymphoblastic leukemia: ICN1; RCT2; CnB1fl/fl T-ALL cells [8], were retrovirally transduced at the same m.o.i. either with the GFP control vector (MIG), or MIG vectors encoding HA-tagged version of either CnB1, or with the constitutive NFAT1 mutants and concomitantly treated with 4OHT to induce CnB1 (PPP3R1) gene deletion and calcineurin inactivation [8] (Fig 4A for a schematic representation of the experiment).