To produce the Nfat-proficient and Nfat-deficient versions of each of these T-ALL, leukemic cells were injected into wild type secondary wild type recipient mice and sub-terminally leukemic mice were treated either with carrier solvent (So) or with tamoxifen (Tam) to induce Cre-mediated deletion of Nfat2 and Nfat4 (see Fig 1A for a scheme of the experiment). This evidence concerns the gene NFATC3 and acute lymphoblastic leukemia.