PIAS2 and Parkinson disease: To determine the effects of PIAS2 overexpression in a human α-syn (hSNCA)-induced familial model of PD, WT mice were injected unilaterally into the nigrostriatal region (SN and STR) with mCherry-only AAV (CTR) and PIAS2-mCherry or an inactive PIAS2C362A-mCherry mutant (mutPIAS2), which carries a mutation in the RING finger-like domain [45], together with hSNCA (Fig. 3f).