To explore the effect of mutations in the S protein of the variants D614G and 501Y.V1 on virus entry into cells expressing dACE2, we constructed three pseudoviruses with S protein from wt SARS-CoV-2, SARS-CoV-2 variants D614G or 501Y.V1, respectively, and tested their infection efficiency. The gene discussed is PROS1; the disease is infection.