qPCR confirmed our UCP-1 immunohistochemistry results and showed significant upregulation of Ucp1 (21.00 ± 0.67 fold), Pgc1a (2.61 ± 0.08 fold), Cidea (3.78 ± 0.31 fold), and Prdm16 (5.15 ± 0.19 fold) gene expression in white fat collected from the BM-hMSC-treated PCOS mice compared with the untreated PCOS mice (Fig. 3j). Here, PRDM16 is linked to polycystic ovary syndrome.