We tested mouse serum using an antibody-based membrane assay and found that these cytokines were significantly lower in the untreated PCOS group (IL-10: 0.50 ± 0.08 fold, INF-γ: 0.79 ± 0.05 fold, TIMP-2: 0.82 ± 0.13 fold) compared with control mice (Fig. 6c). The gene discussed is TIMP2; the disease is polycystic ovary syndrome.