The findings demonstrated the diagnostic capacity of HPRT1 mRNA expression in patients with HNSCC in TCGA cohort [AUC = 0.901, P < 0.0001, 95% confidence interval (CI) = 0.865–0.937; Fig. 3A], GSE107591 cohort (AUC = 0.701, P < 0.019, 95% CI = 0.542–0.859; Fig. 3B) and our validation cohort (AUC = 0.806, P < 0.0001, 95% CI = 0.714–0.897; Fig. 3C). The gene discussed is HPRT1; the disease is head and neck squamous cell carcinoma.