For TUSC3, this analysis utilised the ETV6-RUNX1 positive REH cell line (which expresses TUSC3, as predicted for ETV6-RUNX1 positive ALL cells) and the ETV6-RUNX1 negative NALM6 cell line (in which TUSC3 is hypermethylated and inactivated, as predicted for ETV6-RUNX1 negative ALL cells) (Fig. 3A). This evidence concerns the gene ETV6 and acute lymphoblastic leukemia.