Given the identification of Wnt/β-catenin signaling signatures in association with SPI1 expression in the single-cell data and the presence of a β-catenin binding domain at the N-terminal region of the fusion protein, we next determined whether the N-terminal β-catenin binding domain of TCF1 within the fusion protein is also important in leukemia development. Here, SPI1 is linked to leukemia.