To better understand the functional capability of ILC2 from 3xTg-AD mice, we purified ILC2 from 7-month-old 3xTg-AD mice and control wild-type mice by FACS and cultured them in vitro for 7 days with recombinant growth factors IL-2, IL-33, and IL-7. The gene discussed is IL2; the disease is Alzheimer disease.