Mice are nocturnal animals, and, as expected, baseline radiotelemetry traces show higher BP levels during the night; it is important to note that diurnal BP levels in awake mice (Figure VII in the Data Supplement) are higher than in anesthetized mice (which were measured during the day), but in awake mice differences between groups remained overall comparable to the one measured in anesthesia (Figure 3C, EC=ΔMAP=20 mm Hg; Figure 3D Δ%=20%; RBC ΔMAP=10 mm Hg; Δ%=10%), confirming hypertension in both EC eNOS KO and RBC eNOS KO mice. This evidence concerns the gene NOS3 and hypertensive disorder.