We used the CRISPR-Cas9 gene editing approach to knock in the c.736T>G (p.W246G) mutation that causes human SCA34 [16] into exon 6 of the rat Elovl4 gene (Ensembl: ENSRNOG00000009773) to generate heterozygous Long-Evans F0 founder rats (Fig. 1A). Here, ELOVL4 is linked to spinocerebellar ataxia type 34.