Given the significant increase in the migratory potential observed following JAM-A silencing in our models and prompted by the physiological role of the JAM-A/RAP2c/ROCK1 signalling axis in epithelial barrier function [31–34], we also tested whether loss of JAM-A may affect motility of CRC cells through the ROCK kinase pathway. This evidence concerns the gene ROCK1 and colorectal carcinoma.