MIR21 and colorectal carcinoma: In order to test whether MIR21 overexpression could recapitulate the phenotype observed upon JAM-A modulation in CRC pre-clinical models (Fig. 2A–G), we generated Caco-2 and DLD-1 cell lines carrying a conditional doxycycline-inducible MIR21 construct and confirmed physiological expression of MIR21 following induction with doxycycline in both systems (Supplementary Fig. S6D, E).