In this study, we identified that Tim-1 was abnormally elevated in glioblastoma, and its knockdown inhibited glioblastoma cell proliferation, invasion, and migration via the miR-133a/TGFBR1 axis and the Wnt/β-catenin pathway activation (Additional file 1: Figure S1). Here, TGFBR1 is linked to glioblastoma.