As shown by our results, expressions of β-catenin, Cyclin D1, and c-myc were notably reduced after Tim-1 knockdown or miR-133a upregulation, which could be reversed by TGFBR1 upregulation, which indicated that Tim-1 knockdown inhibited the Wnt/β-catenin pathway activation via the miR-133a/TGFBR1 axis in glioblastoma. The gene discussed is TGFBR1; the disease is glioblastoma.