We aimed to examine the long-term associations between reproductive period, as an indicator of endogenous estrogen, and levels of biomarkers for AD in CSF (Aβ42, P-tau, T-tau, and ratio Aβ42/Aβ40) in a population-based sample of women free from dementia and with natural menopause, followed over 25 years. Here, MAPT is linked to Alzheimer disease.