To further understand whether p53 play any role in RASSF10-mediated nuclear translocation of GADD45a, we performed nuclear cytoplasmic fractionation followed by Western blot analysis in p53-deficient colon cancer cell line HCT116p53−/− with RASSF10 expression under NPM knockdown conditions. The gene discussed is GADD45A; the disease is colonic neoplasm.