In this study, we found that the proliferative activity and colony formation ability of NPC cells were significantly decreased in cells with Notch2 overexpression that were subjected to radiotherapy compared to cells with Notch2 overexpression alone or parental cells subjected to radiotherapy; furthermore, overexpression of Notch2 enhanced radiation-induced G2/M-phase arrest and apoptosis. This evidence concerns the gene NOTCH2 and nasopharyngeal carcinoma.