To address this, we generated Notch2-overexpressing 5–8 F and CNE-2 NPC cells to investigate the efficacy of Notch2 overexpression combined with radiation on cell proliferation, apoptosis, cell cycle progression and the AKT/mTOR signaling pathway with the aim of exploring the underlying mechanisms by which Notch2 overexpression potentially increases the radiosensitivity of NPC cells and providing new ideas for therapeutic strategies for NPC. This evidence concerns the gene NOTCH2 and nasopharyngeal carcinoma.