Figure 2A presented specimens immunostained for p-PI3K and p-Akt. According to the analytic results from immunohistochemistry, the administration of CSWT led to a significant increase in the positive rates of p-PI3K and p-Akt, compared with that of the HF group. Meanwhile, as Figure 2B suggested, based on outcomes from western blotting analyses, the level of p-PI3K(Tyr508)/PI3K and p-Akt(ser473)/Akt were decreased in the HF group while CSWT treatment resulted in remarkable increase of p-PI3K(Tyr508) and p-Akt(ser473) levels. The gene discussed is AKT1; the disease is hydrops fetalis.