The positive expression rates of both p-PI3K and p-Akt showed the lowest number in the HF + LY294002 group, indicating a successful establishment of the PI3K/Akt-inhibition model (p-PI3K: 0.29 ± 0.04; p-Akt: 0.13 ± 0.02). The gene discussed is AKT1; the disease is hydrops fetalis.