Also, E2 prevented ISO-induced vascular endothelial injury, adverse vascular remodeling (excessive thickening of intima-media and interstitial collagen deposition in vascular walls), and hypertension in the rats by decreasing blood pressure and vascular contractility during stress via the homeostatic modulation of the expressions and activities of NO and ET-1(Figure 7). This evidence concerns the gene EDN1 and hypertensive disorder.