In conclusion, the impact of METTL14 on the immune infiltration level of rectal cancer may be related to TGFβ1, TNFSF15, CCL23, CCR10, HLA-G, B2M, and CIN. These findings suggest an interesting possibility that METTL14 expression is associated with anti-PD-1 efficacy. This evidence concerns the gene TNFSF15 and rectal cancer.