The fresh matching specimens were digested to make single-cell suspensions and analyzed by multiparameter flow cytometry; gated on myeloid populations including F4/80+CD24-MHCII+Ly6C- tumor-associated macrophages (TAM’s), CD11bhiLy6CintLy6Ghi neutrophils, CD11bhiLy6ChiLy6G- monocytes, F4/80-CD24+MHCII+Ly6C-CD11b-CD103+ DC1 dendritic cells, and F4/80-CD24+MHCII+Ly6C-CD11b+CD103- DC2 dendritic cells as previously described (18), as well as CD4+ and CD8+ T cells (Figure 2A). This evidence concerns the gene ITGAM and neoplasm.