Zhang et al. (2006) found that the most frequently occurring isoforms of PDK were PDK2 and PDK4. However, PDK4 was not widely validated in its transformation. For exploring the functions and prognostic implication of PDK4 in GC, we conducted conclusive experiments. To evaluate the role of PDK4 in tumorigenesis, we ablated PDK4 expression by siRNAs in human GC cells. Our data indicated that a high expression of PDK4 brought about a short OS rate of patients with GC. Knockdown of PDK4 attenuated the ability of GC cell proliferation, invasion, and metastasis in vitro. Here, PDK4 is linked to gastric cancer.