We found that both p-Nef levels (SMI-312 antibody) and non-phosphorylated Nef levels (SMI-32 antibody) were significantly increased in the cerebellum of SCA3 mice compared to WT animals (t-test, p < 0.001; n = 3 and five pictures per genotype; Figures 9A–D). This evidence concerns the gene S100B and Spinocerebellar ataxia type 3.