Then, the results of KEGG pathway analysis showed that these up-regulated genes participated in cytokine-cytokine receptor interaction, amoebiasis, chemokine signaling pathway, neuroactive ligand-receptor interaction, rheumatoid arthritis, pathways in cancer, transcriptional misregulation in cancer, serotonergic synapse, TNF signaling pathway, chemical carcinogenesis et al. (Figure 3). The gene discussed is TNF; the disease is rheumatoid arthritis.