To verify the above sequencing results, we detected iCAF markers (IL1, IL6, LIF, CSF3) and myCAF markers (Acta2, Ctgf) in CAF treated with CM from shLAMC1 ESCC cells, CAF cocultured with shLAMC1 ESCC cells, and controls. The gene discussed is IL1B; the disease is esophageal squamous cell carcinoma.