Compared with the control group, LAMC1 expression was increased at the protein and mRNA levels in KYSE30 and KYSE450 cells after TGFβ1 treatment, which could be time‐ and concentration‐dependent in ESCC cells (Figs 1H and S2A,B). This evidence concerns the gene TGFB1 and esophageal squamous cell carcinoma.