We found that both CM‐CAF‐pretreated CXCL1 and CM‐CAF‐PBS could promote the proliferation and migration of ESCC cells, and CM‐CAF‐pretreated CXCL1 had a stronger promoting effect than CM‐CAF‐PBS, whereas SB225002 could reverse the effect (Fig. 8B,C). The gene discussed is CXCL1; the disease is esophageal squamous cell carcinoma.