Secondly, using Pearson's correlation analysis of GSE53625 and TCGA data, we found that 1609 genes were positively correlated with TGFβ1 expression in ESCC cancer tissues (r > 0.15, FDR < 0.05) (Figs 1A and S1A). The gene discussed is TGFB1; the disease is esophageal squamous cell carcinoma.