ACTA2 and esophageal squamous cell carcinoma: To verify the above sequencing results, we detected iCAF markers (IL1, IL6, LIF, CSF3) and myCAF markers (Acta2, Ctgf) in CAF treated with CM from shLAMC1 ESCC cells, CAF cocultured with shLAMC1 ESCC cells, and controls.