To further explore the effect of 3-DZNeP on M1/M2 phenotypic shift of alveolar macrophages in the septic ALI/ARDS in vitro, we used LPS (50 ng/ml) and IL-4 (10 ng/ml) to stimulate MH-S cells toward M1 and M2 phenotype differentiation respectively. The gene discussed is IL4; the disease is acute respiratory distress syndrome.