In parallel with our previous finding that 3-DZNeP significantly decreased the inflammatory cytokines in the lung homogenates of CLP-induced ALI mice [10], we noted a marked lower levels of IL-1β, TNF-α and IL-6, and higher levels of IL-10 (Fig. 1C) in the lung tissue on Day 1 post LPS instillation of 3-DZNeP-treated ALI mice comparing with untreated ALI mice and control mice. This evidence concerns the gene TNF and acute respiratory distress syndrome.