Subsequently, we applied ssGSEA algorithm in R package GSVA to preliminarily estimate the relationships between the expression of ARNTL2 and its top 3 co-expressed genes and tumor infiltrating immune cell types, as shown in Fig. 6d, ARNTL2 and its co-expressed genes ARPC2, GNB4, CAPZA1 were remarkably associated with the infiltrating level of T helper cells, macrophages, T cells, B cells and dendritic cells (DC) (P < 0.001), while showed weak associations with neutrophils and mast cells (P < 0.01). This evidence concerns the gene GNB4 and neoplasm.