To optimize the screening protocol for assessing cell lines’ sensitivity to pharmacological inhibition or genetic inactivation via CRISPR/Ca9-mediated gene editing, we selected 20 non-small cell lung carcinoma (NSCLC) cell lines with wild-type or mutant EGFR in the BMS-PRISM collection (Supplementary Table 1). The gene discussed is EGFR; the disease is non-small cell lung carcinoma.