In this study, we successfully established and identified iRGD‐modified exosomes (iRGD‐exosome), and it was confirmed that both αvβ3 and NRP‐1 were highly expressed in colon cancer and cell lines, which demonstrated that colon cancer could be a potential target for iRGD‐exosome. The gene discussed is NRP1; the disease is malignant colon neoplasm.