To further throw light on the differential functional gene sets in AML, gene set variation analysis was performed, and 15 activated GO terms were significantly enriched in MSigDB_C5_GO (Table 1) and 10 significantly pathways were found in C2 curated gene sets (Table 2), such as ‘co-stimulation by the CD28 family’, ‘RADMACHER AML prognosis’, and ‘REACTOME FLT3 signaling’. Here, CD28 is linked to acute myeloid leukemia.