Here, we used BV2 microglial cells co-transfected with LRRK2-Wt expression plasmid and pre-miR-335, and further incubated with LPS for additional 24 h; and SH-SY5Y neuroblastoma cells, with or without α-syn overexpression co-transfected with LRRK2-Wt and pre-miR-335. The gene discussed is LRRK2; the disease is neuroblastoma.