They optimized the length and position of ASO by using microwalk, finally found that ASO10-27 can target the intronic splicing silencer-N1 (ISS–N1) element on intron 7 to prevent the binding of the splicing inhibitor HNRNPA1/2, thereby increasing the inclusion of exon 7 MOE P = S modified ASO10-27 (nusinersen once used its name), improved motor function in SMA mouse models, and extended the life span of SMA mice by 25 times [25, 62–64]. The gene discussed is HNRNPA1; the disease is proximal spinal muscular atrophy.