Moreover, TSC2 mutations (3/11, 27.3%) was the most frequently mutated branchy driver, while sporadically mutated branchy drivers, such as CCND1, CDKN2A, LRP1, MAP3K1 and PTEN, were also observed in multifocal HCC, which were supposed to drive the complicated heterogeneity and multiple phenotypes in HCC. This evidence concerns the gene CDKN2A and hepatocellular carcinoma.