To understand the functional role of Wnt5a in vitro, RA td-FLS were stimulated with Wnt5a for 4 h and 24 h.Our data showed that Wnt5a enhanced rapidly the expression of IL1β, IL6, IL8, CCL2, CXCL10 and COX2 in RA td-FLS. The gene discussed is CXCL8; the disease is rheumatoid arthritis.