Single-cell suspensions from spleen, liver or bone marrow were determined using flow cytometry analysis, and the results showed that the percentages of GFP+ leukemia cells in these organs from NLRP3-GFP AML mice were significantly higher than those from Ctrl-GFP AML mice (Figure 2D, Supplementary Figure 2G). Here, NLRP3 is linked to acute myeloid leukemia.