ALPK2 and urinary bladder cancer: In order to explore the role played by ALPK2 in the development and progression of bladder cancer, we prepared lentiviral constructs expressing shRNAs targeting ALPK2 (shALPK2-1 and shALPK2-2) or shCtrl (as a negative control); these constructs were then used to transfect EJ and T24 cells.