In regard to TIL production, we did not observe any benefit from pretreating tumors with vem for 7 days before excision, that is, young TIL culture days, number of TILs infused, CD4/CD8 composition in infusion product, TIL anti-tumor reactivity, compared with what we have previously reported6 and another TIL study performed in parallel at our center.48 In the study reported by Deniger et al,47 an additional tumor was excised for immunological studies after 2 weeks of vem treatment in all patients. The gene discussed is CD8A; the disease is neoplasm.