Considering the above notions, we accomplished a thorough analysis of the secreted metabolome and of the cellular proteome of primary astrocytes from the spinal cord of newborn Tg mice expressing the ALS-related hSOD1(G93A) or the non-pathogenic wild-type (WT) hSOD1 using non-targeted metabolomics and proteomics approaches. The gene discussed is SOD1; the disease is amyotrophic lateral sclerosis.