Splenic CD68+ macrophages and CD11c+ DCs from tumor-bearing mice (tumor-bearing Mφs or DCs), cryo-thermal group (cryo-thermal Mφs or DCs) and DFO treated cryo-thermal group (cryo-thermal + DFO Mφs or DCs) were sorted by using magnetic beads and cocultured for 24 h with CD4+ T cells derived from tumor-bearing mice 24 days after inoculation. This evidence concerns the gene CD68 and neoplasm.