IFN-γ, IL-10, TNF-α, IL-5, IL-12p70, IL-13, GM-CSF, G-CSF, IL-15, VEGF-A, IFN-α, IL-9, IL-23, IL-8, IL-17A and IP-10 were under the LLOQ in all fecal extracts (ALS and controls). The gene discussed is IFNG; the disease is amyotrophic lateral sclerosis.