In silico analysis included the following genes: AKT1, AKT2, AKT3, CHUK, GSK3β, EGFR, PTEN, and PIK3AP1. Analysis of CNA in a total sample of diffuse brain gliomas showed that the highest total number of CNA was confined to the GBM group, while the lowest CNA of all tested genes was found in the DA group. This evidence concerns the gene AKT3 and glioblastoma.