Palmitate (0.8 mM and 0.6 mM) induced insulin resistance in C2C12 myotubes, suppressed the insulin-stimulated phosphorylation of downstream target proteins, increased AA concentration (all p values < 0.0001), increased pro-inflammatory cytokines (TNF-α and IL6), and reduced GLUT4 mRNA expression. The gene discussed is INS; the disease is Insulin resistance.