Loss of activity of the caged drug and recovering the activity upon UV irradiation were further addressed in in vitro tubulin polymerization (porcine brain tubulin) and tubulin binding assays (competitive colchicine binding assay), as well as via assessing the effect on the tubulin network and cell morphology in U251 glioblastoma cells (immunofluorescence staining of β-tubulin) and measuring the apoptotic effect on RN1 glioblastoma cells (Annexin V staining). The gene discussed is ANXA5; the disease is glioblastoma.