PRNP and prion disease: Based on the fact that prion diseases are fatal and untreatable, and that PrPC is essential for the spreading of PrPSc, Kang and collaborators (2018) [94] decided to investigate PrPC suppression by an artificial dual miRNA (DmiR) and its effects in pathogenic prion replication in C2C12 myoblasts and primary mixed neuronal and glial cells culture (MNGC).