CD40LG and coinfection: When the prevalence was stratified as per the diagnostic tests for CHIKV infection, the prevalence of co-infection was 10% (95% CI: 8–11%, I2: 99.5%) using RDT (IgM), 7% (95% CI: 4–10%) using PRNT, 1% (95% CI: 0–2%, I2: 41.5%) using IgM and IgG ELISA, and 4% (95% CI: 2–6%) using real-time RT-PCR (Figure 3).