CD40LG and malaria: When the prevalence was stratified as per diagnostic tests for CHIKV infection, the prevalence of CHIKV infection in malaria patients was 39% (95% CI: 34–44%, I2: 99.7%) using RDT (IgM), 43% (95% CI: 30–57%) using PRNT, 5% (95% CI: 3–7%, I2: 5.18%) using IgM and IgG ELISA, and 9% (95% CI: 6–15%) using real-time RT-PCR (Figure 6).