Endothelial cytoskeleton rearrangements have been correlated with cancer cell transmigration, being particularly described during intravasation through vascular ECs, where an endothelial actomyosin circumferential pore is formed, regulated by MLCK phosphorylation of MLC and prompting cytoskeleton rearrangements and actin fibre formation [28,61]. The gene discussed is MYLK; the disease is cancer.